Functional complementation by electroporation of human BACs into mammalian fibroblast cells.
نویسندگان
چکیده
Bacterial Artificial Chromosomes (BACs) have been used to complement a metabolic defect and to transfer a drug resistance marker into mammalian cells by electroporation. The selectable markers are stable and the recipient cells have BAC DNA integrated into the chromosomes as shown by fluorescent in situ hybridization, PCR and Southern hybridization.
منابع مشابه
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عنوان ژورنال:
- Nucleic acids research
دوره 26 4 شماره
صفحات -
تاریخ انتشار 1998